Molecular Cancer Therapeutics
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Vol. 1, 295-302, March 2002     Molecular Cancer Therapeutics
© 2002 American Association for Cancer Research

Antiangiogenic Effect by SU5416 Is Partly Attributable to Inhibition of Flt-1 Receptor Signaling 1

Takashi Itokawa, Hiroki Nokihara, Yasuhiko Nishioka, Saburo Sone, Yukihide Iwamoto, Yuji Yamada, Julie Cherrington, Gerald McMahon, Masabumi Shibuya, Michihiko Kuwano and Mayumi Ono2

Departments of Medical Biochemistry [T. I., M. K., M. O.], Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan Orthopedic Surgery [Y. I.], Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan Third Department of Internal Medicine, University of Tokushima, Tokushima 770-8583, Japan [H. N., Y. N., S. S.] Cancer Research Laboratory, Taiho Pharmaceutical Co., Saitama 357-8527, Japan [Y. Y.] SUGEN, Inc., South San Francisco, California 94080 [J. C., G. M.] Department of Genetics, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan [M. S.]


   Abstract
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
Interaction between vascular endothelial growth factor (VEGF) and its cognate receptors, KDR/Flk-1 and Flt-1, of vascular endothelial cells is expected to induce an angiogenesis "switch" in tumors and other angiogenesis-associated diseases. SU5416, a selective inhibitor of the KDR/Flk-1 tyrosine kinase, is known to be a potent inhibitor of tumor angiogenesis. In this study, we first observed that SU5416 inhibited Flt-1 tyrosine kinase activity at similar doses, in addition to inhibiting KDR/Flk-1 tyrosine kinase activity in response to VEGF. SU5416 inhibited cell migration of human vascular endothelial cells expressing both Flt-1 and KDR in response to VEGF and also inhibited the cell migration in response to placenta growth factor (PlGF), a specific ligand for Flt-1. Chemotaxis of monocytes expressing only Flt-1 was also inhibited by SU5416 in a dose-dependent manner. Moreover, SU5416 was found to inhibit tyrosine kinase of Flt-1 in response to PlGF in vitro. And angiogenesis induced by PlGF in a Matrigel plug assay was inhibited by administration of SU5416. The antiangiogenic effects by this VEGF receptor-targeting compound appeared to be mediated through interference not only with KDR/Flk-1 but also with Flt-1. Cell migration of vascular endothelial cells and monocytic cells through Flt-1, thus, might play a key role in VEGF-induced tumor angiogenesis in concert with KDR/Flk-1.


   Introduction
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
VEGF, 3 one of the most potent angiogenic factors, regulates endothelial cell proliferation and vascular permeability (1, 2). Disruption of the mouse VEGF gene induces abnormal blood vessel development and lethality in embryonic maturation. Two VEGF receptor tyrosine kinases, Flt-1 (3) and KDR/Flk-1 (4), have been identified. Disruption of the murine Flk-1 gene results in a severe deficiency in vasculogenesis and production of hematopoietic cells (blood islands) during embryogenesis (5). In contrast, Flt-1 homozygous gene disruption results in an abundance of endothelial cells with abnormal vasculature. This suggests that Flt-1 plays an essential role in both vasculogenesis and angiogenesis (6). Hiratsuka et al. (7) reported that Flt-1 tyrosine kinase-deficient mice appeared to develop normal vessels, although VEGF-induced macrophage migration was attenuated. On the other hand, Hiratsuka et al. (8) subsequently reported that tyrosine kinase of Flt-1 was important for angiogenesis under certain pathological conditions, such as tumor angiogenesis. Carmeliet et al. (9) have further studied the role of PlGF, a specific ligand for Flt-1 (10, 11), and reported that vascular endothelial cells amplify their responsiveness to VEGF during the "angiogenesis switch" in many pathological disorders through up-regulation of PlGF-Flt-1 receptor signaling. An Flt-1-mediated signal elicits cell migration through actin reorganization via the activation of p38 mitogen-activated protein kinase in HUVE cells (12). Both KDR/Flk-1 and Flt-1, thus, appear to be required for angiogenesis under pathological conditions, including cancer, as well as vasculogenesis during embryonic development.

On the other hand, tumor enlargement often depends on angiogenesis, and VEGF and its receptors play an essential role in tumor angiogenesis (13). VEGF is secreted by tumor cells and by tumor-associated stromal cells, including endothelial cells, fibroblasts, and macrophages. Thus, both VEGF produced by the surrounding stroma (14) and that produced by tumor cells themselves (15) appear to play a key role in tumor angiogenesis. VEGF and its receptor molecules have been implicated in angiogenesis of various human tumor types (13, 16).

Since the initial report that angiogenesis is closely correlated with metastasis or prognosis in patients with breast cancer (17), numerous studies have consistently demonstrated that angiogenesis plays a major role in the malignant behavior of various tumor types such as breast cancer and glioblastoma and also in many other angiogenesis-associated diseases (13, 16, 18). Agents that specifically target tumor angiogenesis are being developed and are expected to modulate not only tumor enlargement but also metastasis or invasion in various malignant tumors (19). Furthermore, because both KDR/Flk-1 and Flt-1 are expressed rather preferentially in vascular endothelial cells (20), development of tyrosine kinase inhibitors that target both KDR/Flk-1 and Flt-1 is expected to inhibit neovascularization in malignant tumors. A synthetic antiangiogenesis compound, SU5416, was developed to target KDR/Flk-1 receptor kinase. Fong et al. (21) reported that SU5416 exerts its activity through inhibition of KDR/Flk-1 receptor kinase and that SU5416 showed antitumor activity in an experimental animal model system of tumor growth. Shaheen et al. (22) reported that SU5416 inhibited the growth of colon cancer metastasis in the liver. In this study, we first demonstrated that SU5416 also inhibited Flt-1 tyrosine kinase and that Flt-1 tyrosine kinase is required for cell migration by endothelial cells and also by monocyte/macrophages. The antiangiogenic activity of SU5416 thus appears to be because of inhibition of not only KDR/Flk-1 but also Flt-1 tyrosine kinase.


   Materials and Methods
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Materials.
Human VEGF165, human PlGF-1, and mouse PlGF-2 were purchased from R&D Systems (Minneapolis, MN). SU5416 (chemical name: 3-[(2,4-dimethylpyrrol-5-yl) methylidenyl]-iodolin-2-one) was provided from SUGEN (21, 23) and Taiho Pharmaceutical Co. A monoclonal antibody specific to phosphotyrosine (PY-20) was purchased from ICN Biochemicals (Cosa Mesa, CA). Anti-Flt-1 antibody and anti-KDR antibody were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Low growth factor Matrigel was purchased from Becton Dickinson (Bedford, MA).

Cell Lines and cDNA Transfection.
A mouse NIH3T3 cell line overexpressing human KDR receptor was established previously (24) and was designated as NIH3T3/KDR for the present study. Flt-1 expression vector BCMGS neo Flt-1 (25) was transfected into NIH3T3 cells followed by selection of G418-resistant clones to establish NIH3T3/Flt-1. Mouse renal cell carcinoma RENCA cells were kindly provided by Dr. I. J. Fidler and cultured in RPMI supplemented with 10% FBS, 60 µg/ml penicillin, and 60 µg/ml kanamycin. HUVE cells were purchased from Clonetics Co. (Walkersville, San Diego, CA) through Sanko Junyaku Co., Ltd. (Tokyo, Japan) and cultured in endothelial cell basal medium (EBM)-2 supplemented with 2% FBS.

Mouse Dorsal Air Sac Assay.
A dorsal air sac assay was performed in 7–10-week-old male mice according to a method published previously (26, 27). RENCA cells (1 x 106 cells) were suspended in PBS and injected into a chamber consisting of a ring (Millipore Corp., Bedford, MA) covered with Millipore filters (0.45-µm pore size) on both sides. This chamber containing RENCA cells was implanted into a dorsal air sac produced in the mouse by injecting 10 ml of air. After implantation, SU5416 (25 mg/kg/day) was administered by i.p. injection. Five mice in each group were killed and carefully skinned on day 5. After the implanted chamber was removed from the s.c. air fascia, a ring without a filter was placed on the same site and photographed (27). We counted the number of meandered blood vessels within the chamber in the area of the air sac fascia. The angiogenic response was graded as 0, 1, 2, 3, 4, or 5 according to the number of newly curled thin blood vessels (zero, one, two, three, four, or more than five vessels, respectively).

Immunoblotting Assay.
Confluent NIH3T3/Flt-1 or NIH3T3/KDR cells were cultured in serum-depleted DMEM for 48 h. The cells were then preincubated with SU5416 at concentrations ranging from 0.05 to 5 µM for 2 h followed by stimulating with 20 ng/ml VEGF for 5 min at 37°C. The cells were then rinsed with ice-cold PBS and lysed in lysis buffer (50 mM HEPES, 150 mM NaCl, 1% Triton X-100, and 10% glycerol containing 1 mM phenylmethylsulfonyl fluoride, 10 µg/ml aprotinin, 10 µg/ml leupeptin, and 1 mM sodium vanadate). Cell lysates were subjected to SDS-PAGE and transferred onto Immobilon membranes (Millipore Corp.). After transfer, blots were incubated with the blocking solution and probed with antiphosphotyrosine (PY20) antibody, anti-Flt-1 receptor antibody, or anti-KDR antibody followed by washing. The protein contents were visualized using horseradish peroxidase-conjugated secondary antibodies followed by enhanced chemiluminescence (Amersham).

In Vitro Kinase Assay.
Immunoprecipitated Flt-1 was washed four times with lysis buffer and twice with kinase assay buffer [20 mM HEPES (pH 7.4), 10 mM MgCl2, 2 mM, MnCl2, 0.05% Triton X-100, and 1 mM DTT]. The agarose beads used for precipitations were suspended in kinase assay buffer containing 0.25 µCi/µl [{gamma}-32P] ATP and then incubated on ice for 10 min. The beads were then washed three times with kinase assay buffer, suspended in sample buffer, and heated for 5 min at 100°C. For SDS-PAGE, the gel was fixed in methanol:acetic acid, treated with 1 M KOH for 30 min at 55°C, fixed again, and examined by autoradiography.

Isolation of Human Monocytes and Chemotactic Activity.
Human leukocytes from peripheral blood (200 ml) of healthy donors were collected, and mononuclear cells were separated using a lymphocyte separation medium (Litton Bionetics, Kensington, MD) and Beckman JE-5.0 elutriation system as described previously (28). Fractions enriched in monocytes (95%) were obtained at 3000 rpm and flow rates of 30–36 ml/min. More than 97% of the cells were viable. Monocyte chemotactic activity was measured using a 48-well chemotaxis chamber (Neuro Probe, Cabin John, MD) with a 5-µm pore-size polycarbonate filter as described previously (29).

VEGF or PlGF-induced Migration Assay of HUVE Cells.
Cell migration assay was performed using a 24-well chamber with 1.33 µg/ml fibronectin-coated 8-µm polycarbonate filters (30). HUVE cells (3 x 105 cells) were suspended in EBM-2 containing 0.5% FBS and seeded in the inner chamber. In the outer chamber, serial dilutions of SU5416 with or without VEGF (20 ng/ml) in the same medium were added. After incubation for 3 h at 37°C, nonmigrated cells on the upper surface of the filter were removed, and the cells that had migrated under the filter were counted manually by examination under the microscope (31).

Mouse Matrigel Plug Assay.
The mouse Matrigel plug assay was performed as described previously (32). In brief, 0.3 ml of Matrigel supplemented with 400 ng of PlGF was s.c. inoculated into BALB/c mice. After the inoculation, SU5416 (25 mg/kg/day) was administered by i.p. injection. Four days after the inoculation, the mice were sacrificed, and the gels were removed by retaining the overlying skin. These gels were then fixed in 10% buffered-formalin, embedded in paraffin, and sectioned at a 4-µm thickness. The sections were then deparaffinized and stained with Masson-Trichrome. The numbers of neovasculatures within the gels on day 4 were counted (32).


   Results
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
SU5416 Inhibits Tumor-induced Angiogenesis in Vivo.
Fong et al. (21) have reported previously that SU5416 shows antitumor activity against mice bearing various tumor types. To confirm this previous finding, we examined whether SU5416 could also inhibit tumor angiogenesis in an experimental model system assessed by a dorsal air sac assay in vivo. Implantation of a chamber containing murine renal cell carcinoma RENCA cells, which produce VEGF in the dorsal air sac, resulted in development of microvessels (as indicated by arrows) with curled thin structures and many tiny bleeding spots in addition to the preexisting vessels (Fig. 1A). Administration of SU5416 at 25 mg/kg/day for 5 days markedly reduced the development of such tumor cell-induced microvasculatures. However, treatment with SU5416 did not affect any preexisting vessels. The quantitative analysis demonstrated a significant reduction of neovascularization by administration of SU5416; neovascularization grades were 5 ± 0.2 and 1.8 ± 0.4 in the absence and presence of SU5416, respectively. SU5416 at 25 mg/kg/day was found to be effective against tumor-induced angiogenesis in vivo (Fig. 1), in agreement with the previous study (21).



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  		Fig. 1. SU5416 inhibits tumor-induced angiogenesis in vivo. Inhibition of tumor angiogenesis by RENCA cells in the mouse dorsal air sac assay after systemic treatment with SU5416. Representative photographs of the mouse dorsal air sac assay, RENCA cells in untreated mice (A), and in mice treated with SU5416 at 25 mg/kg/day (B) are shown. pv, preexisting vessels; tn, tumor neovasculature.

 
SU5416 Inhibits Autophosphorylation of Flt-1, KDR, and Tyrosine Kinase Activity in Response to VEGF.
Consistent with the previous study by Fong et al. (21), immunoblot analysis with antiphosphotyrosine antibody (PY20) after receptor activation by VEGF showed that SU5416 at >=0.5 µM inhibited autophosphorylation of KDR receptor in its cDNA transfectant, NIH3T3/KDR with IC50 of 0.1 µM (Fig. 2A). In NIH3T3/Flt-1 cells, immunoblotting with PY20 indicated that only the upper of the two bands detected as Flt-1 was phosphorylated specifically in response to VEGF with IC50 of 0.1 µM(Fig. 2B). Flt-1 autophosphorylation was enhanced by exogenous addition of VEGF. SU5416 at >=0.5 µM almost completely inhibited the Flt-1 autophosphorylation (Fig. 2B). However, this compound did not affect cellular levels of Flt-1 receptor protein. We also examined whether SU5416 could inhibit in vitro kinase activity by Flt-1. As seen in Fig. 2C, its in vitro kinase activity was markedly blocked by >=0.5 µM doses of SU5416. SU5416 could thus inhibit tyrosine kinase activity of Flt-1, as well as KDR/Flk-1.



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  		Fig. 2. Effect of SU5416 on VEGF-induced KDR and Flt-1 receptor autophosphorylation and kinase activity of Flt-1. NIH3T3/KDR and NIH3T3/Flt-1 cells were grown to near confluence and then serum depleted for 48 h. The cells were preincubated with indicated concentrations of SU5416 for 2 h, followed by the addition of 20 ng/ml VEGF for 5 min at 37°C. Protein extracts were resolved by 7.5% SDS-PAGE and probed with antiphosphotyrosine (A; PY20) antibody, anti-KDR receptor antibody and antiphosphotyrosine (B; PY20) antibody, or anti-Flt-1 receptor antibody (C). In vitro kinase assay of Flt-1 receptor activity. Flt-1 was immunoprecipitated from equal amounts of cell lysates and incubated with [{gamma}-32P] ATP. 32P-labeled Flt-1 was detected by autoradiography.

 
SU5416 Inhibits PlGF-induced Flt-1 Autophosphorylation.
PlGF is a specific ligand for Flt-1 but not for KDR/Flk-1 (10, 11). We next examined whether PlGF-induced tyrosine autophosphorylation of Flt-1 would be blocked. Treatment of NIH3T3/Flt-1 cells with 50 ng/ml PlGF enhanced the autophosphorylation of Flt-1 (Fig. 3) but not that of KDR (data not shown). SU5416 at 0.5–5 µM almost completely blocked the Flt-1 tyrosine autophosphorylation in response to PlGF (Fig. 3). SU5416 thus inhibited both PlGF- and VEGF-stimulated Flt-1 tyrosine autophosphorylation. Fig. 3 also shows that the upper and lower bands of Flt-1 are phosphorylated by PlGF and that phosphorylation of both bands is inhibited by similar doses of SU5416. It remains unclear why the lower band of Flt-1 is phosphorylated by PlGF but not by VEGF (Figs. 2B and 3).



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  		Fig. 3. Effect of SU5416 on PlGF-induced Flt-1 receptor autophosphorylation. NIH3T3/Flt-1 cells were grown to near confluence and then serum depleted for 48 h. The cells were preincubated with increasing concentrations of SU5416 for 2 h followed by the addition of 50 ng/ml PlGF for 5 min at 37°C. Protein extracts were resolved by 7.5% SDS-PAGE and probed with antiphosphotyrosine (PY20) antibody or anti-Flt-1 receptor antibody.

 
SU5416 inhibits both migration of vascular endothelial cells and chemotaxis of human monocytes in response to VEGF or PlGF.

We next examined how inhibition of Flt-1 kinase activity and/or KDR/Flk-1 kinase activity can modulate the migration of vascular endothelial cells and monocytes. VEGF is well known to stimulate migration of HUVE cells through its interaction with its cognate receptor KDR/Flk-1 and/or Flt-1 (12). In contrast, PlGF is known to be a ligand and induce autophosphorylation of Flt-1 (33). We assumed that inhibition of Flt-1 tyrosine kinase activity could abrogate the specific signal transduction by PlGF.

As shown in Table 1, VEGF or PlGF stimulated cell migration of HUVE cells ~2- or 1.5-fold, respectively. And this VEGF- or PlGF-specific migration was almost completely blocked by SU5416 at >=0.5 µM.


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  		Table 1 The effect of SU5416 on cell migration of vascular endothelial cells by VEGF or PlGFa

 
Chemotaxis of human monocytes is known to be enhanced in response to C-C chemokines, MCP-1 and macrophage inflammatory protein-1{alpha}, as well as in response to VEGF (34). As shown in Table 2, VEGF induced a 2-fold and MCP-1 induced a 5-fold increase in the chemotaxis of human monocytes. As monocytes express only Flt-1, the VEGF-stimulated chemotaxis is Flt-1 tyrosine kinase dependent. The VEGF-stimulated chemotaxis of monocytes was almost blocked completely by SU5416 at >=5 µM. However, even at 5 µM, SU5416 could not inhibit MCP-1-stimulated chemotaxis activity of monocytes. Three independent experiments using different monocyte preparations yielded data comparable with that in Table 2.


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  		Table 2 Effect of SU5416 on VEGF- or MCP-1-induced chemotaxis of human monocytesa

 
SU5416 Inhibits PlGF-induced Angiogenesis in Vivo.
Finally, we examined whether SU5416 inhibits PlGF-induced angiogenesis using a Matrigel plug assay. Angiogenesis was enhanced in response to PlGF (Fig. 4B). Administration (i.p.) of SU5416 (25 mg/kg/day) for 5 days resulted in marked inhibition of PlGF-induced angiogenesis in Matrigel (Fig. 4C). Quantitative analysis demonstrated that SU5416 significantly reduced PlGF-induced neovascularization: Lumen numbers were estimated to be 6.6 ± 1.5, 20.8 ± 7.2, and 6.5 ± 3 in the control (Matrigel alone), 400 ng of PlGF and SU5416-treated groups, respectively (Fig. 4D). The mouse Matrigel plug assay thus demonstrated that SU5416 inhibited PlGF-induced angiogenesis in vivo.



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  		Fig. 4. Inhibition of PlGF-induced angiogenesis by SU5416 in mouse Matrigel plug assay. The Matrigel was removed, stained with Masson-Trichrome, and analyzed histologically at 4 days after inoculation. The photographs show Matrigel alone (A), Matrigel containing 400 ng of PlGF (B), and Matrigel containing 400 ng of PlGF with i.p. administration of SU5416 at 25 mg/kg/day (C; x400). Lumen number in Matrigel was quantified when four areas were determined. Mean number ± SD (D). *, significant difference between VEGF alone and VEGF with SU5416 (P < 0.01).

 

   Discussion
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
SU5416 is a potent antiangiogenic agent that was developed by targeting one of the VEGF receptors, KDR/Flk-1, and that has demonstrated antitumor activity in mice bearing various tumor types (21, 22). In the present study, we also observed marked inhibition of VEGF-induced angiogenesis of mouse cornea (data not shown) and development of microvessels induced by implanted tumor cells in the dorsal air sac in mice when SU5416 was administered for 5 days. SU5416 inhibits KDR/Flk-1 kinase through its binding to the conserved ATP-binding site within the kinase domain of the receptor (21). We demonstrated that SU5416 at 0.5–5 µM inhibited not only KDR/Flk-1 receptor tyrosine kinase but also Flt-1 receptor tyrosine kinase in response to VEGF. Moreover, we also demonstrated that SU5416 at 0.5–5 µM could inhibit Flt-1 receptor tyrosine kinase in response to PlGF that bind only to Flt-1 receptors.

VEGF markedly stimulated cell migration of vascular endothelial cells, and this VEGF-induced migration was inhibited by SU5416. Because HUVE cells express both VEGF receptors, it remains unclear which receptor is responsible for SU5416-induced inhibition of vascular endothelial cell migration promoted by VEGF. Kanno et al. (12) have reported that DNA synthesis in VEGF-stimulated HUVE cells is mediated preferentially by KDR/Flk-1 and also that the migration of VEGF-stimulated HUVE cells is mediated by both Flt-1 and KDR/Flk-1 in a complex way. Inhibition of HUVE cell migration by SU5416 thus might be because of both VEGF receptors. On the other hand, Ziche et al. (35) demonstrated that PlGF was as effective as VEGF in promoting cell migration of endothelial cells, although slightly less potent. Consistent with this report, cell migration of HUVE cells was stimulated ~1.5-fold in response to PlGF, and this PlGF-induced migration by HUVE cells was also blocked in the presence of SU5416. Moreover, we demonstrated that SU5416 inhibited VEGF-induced chemotaxis of monocytes, which express only Flt-1. These results suggest that PlGF-induced migration of endothelial cells and VEGF-induced chemotaxis of monocytes are mediated through Flt-1 kinase.

In our present study, angiogenesis was induced in Matrigel in response to PlGF. Administration of SU5416 for 5 days resulted in successful inhibition of PlGF-induced angiogenesis as revealed by a mouse Matrigel plug assay. SU5416 thus could block angiogenesis through its inhibition of PlGF-Flt-1 receptor signaling. It has been reported that blood vessels of larger sizes (>100 µm in diameter) were developed when PlGF-expressing cancer cells were transplanted in Flt-1 tyrosine kinase-deficient homozygous Flt-1 TK-/- mice, whereas vessels of smaller sizes (<50 µm in diameter) were developed when VEGF-expressing cancer cells were transplanted in Flt-1 TK-/- mice (7). This suggests that the angiogenesis pathway induced by VEGF could be different from that induced by PlGF. Carmeliet et al. (9) have reported recently that embryonic angiogenesis is not affected by deficiency of PlGF. However, the loss of PlGF impaired plasma extravasation and collateral growth during ischemia and angiogenesis under various pathological conditions, such as inflammation, wound healing, and cancer (9). In concert with VEGF, PlGF thus appears to play a key role in angiogenesis under pathological conditions, including cancer. SU5416 is thus expected to show its antiangiogenesis activity in vivo, possibly through a blocking of the angiogenesis pathway by not only KDR/Flk-1 but also Flt-1.

On the other hand, we also observed that SU5416 inhibited the human monocyte migration induced by VEGF. It remains uncertain whether this inhibitory effect is involved in the drug-induced antiangiogenesis in vivo. In a study by Hiratsuka et al. (7), VEGF-induced chemotaxis of macrophages was abolished when macrophages were derived from Flt-1 TK-/- mice. Activated monocytes/macrophages in the tumor stroma often produce angiogenic factors and proteolytic enzymes and modulate angiogenesis in the tumor environment (18, 36–38). Macrophage infiltration correlates with angiogenesis or malignancy in human breast cancers (39), human gliomas (40), renal cell carcinomas (41), and human melanomas (42). Infiltration of thymidine phosphorylase-positive (43, 44), hemeoxygenase1-positive, or Cap43-positive monocytic (40, 41) cells is also closely associated with angiogenesis or prognosis in human gliomas and renal cell carcinomas. The inhibition of monocytic/macrophage infiltration in tumor stroma thus might play a key role in angiogenesis inhibition in some tumor types. Hiratsuka et al. (8) have reported recently the role of Flt-1 tyrosine kinase in pathological angiogenesis in Flt-1 TK-/- mice. They observed no apparent differences in the number of infiltrating macrophages in transplanted Lewis lung carcinoma (LLC)-PlGF-induced tumor at day 8. On day 16, however, the number of infiltrated macrophages were 3-fold larger than in Flt-1 TK+/+ mice than TK-/- mice. This suggests that PlGF might not play a major role in infiltration of macrophages in the model system. However, it still remains unclear how infiltration of monocytes/macrophages might be associated with angiogenesis. In the present study, no macrophage infiltration was observed in Matrigel plug assay. Additional in vivo study with any adequate assay model will be needed to determine whether SU5416 can affect the infiltration of macrophages in association with angiogenesis.

In conclusion, a potent antiangiogenesis and antitumor agent, SU5416, was developed by targeting KDR/Flk-1 tyrosine kinase. We demonstrated that SU5416 could inhibit Flt-1 tyrosine kinase, as well as KDR/Flk-1 tyrosine kinase. SU5416 also could inhibit not only migration of vascular endothelial cells in response to PlGF or VEGF but also migration of monocytic cells in response to VEGF in vitro. Moreover, SU5146 could inhibit angiogenesis in Matrigel induced by PlGF in vivo. Our results strongly suggest that the antitumor and antiangiogenesis effects of SU5416 are because of inhibition of not only KDR/Flk-1 but also Flt-1 tyrosine kinases.


   Acknowledgments
 
We thank Drs. Soh-ichiro Ogawa, Masahiro Okamoto, and Laura K. Shawver for fruitful discussion and Dr. Shigeru Kanda for technical assistance.


   Footnotes
 
1 Supported by grant-in-aid for Cancer Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan. Back

2 To whom requests for reprints should be addressed, at Department of Medical Biochemistry, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Phone: 81-92-641-6100; Fax: 81-92-642-6203; E-mail: mayumi{at}biochem1.med.kyushu-u.ac.jp. Back

3 The abbreviations used are: VEGF, vascular endothelial growth factor; PlGF, placenta growth factor; HUVE, human umbilical vascular endothelial; MCP-1, monocyte chemoattractant protein-1; FBS, fetal bovine serum; KDR, kinase insert domain containing receptor; Flk-1, fetal liver kinase-1; Flt-1, fms-like tyrosine kinase-1. Back

Received 12/10/01; revised 1/31/02; accepted 2/ 4/02.


   References
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 

  1. Keck, P. J., Hauser, S. D., Krivi, G., Sanzo, K., Warren, T., Feder, J., and Connolly, D. T. Vascular permeability factor, an endothelial cell mitogen related to PDGF. Science (Wash. DC),246:1309 –1312,1989 .[Abstract/Free Full Text]
  2. Leung, D. W., Cachianes, G., Kuang, W. J., Goeddel, D. V., and Ferrara, N. Vascular endothelial growth factor is a secreted angiogenic mitogen.Science (Wash. DC),246:1306 –1309,1989 .[Abstract/Free Full Text]
  3. Shibuya, M., Yamaguchi, S., Yamane, A., Ikeda, T., Tojo, A., Matsushime, H., and Sato, M. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.Oncogene,5:519 –524,1990 .[Medline]
  4. Terman, B. I., Dougher-Vermazen, M., Carrion, M. E., Dimitrov, D., Armellino, D. C., Gospodarowicz, D., and Bohlen, P. Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor. Biochem. Biophys. Res. Commun.,187:1579 –1586,1992 .[CrossRef][Medline]
  5. Shalaby, F., Ho, J., Stanford, W. L., Fischer, K. D., Schuh, A. C., Schwartz, L., Bernstein, A., and Rossant, J. A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis. Cell,89:981 –990,1997 .[CrossRef][Medline]
  6. Fong, G. H., Rossant, J., Gertsenstein, M., and Breitman, M. L. Role of the Flt-1 receptor tyrosine kinase in regulating the assembly of vascular endothelium. Nature (Lond.),376:66 –70,1995 .[CrossRef][Medline]
  7. Hiratsuka, S., Minowa, O., Kuno, J., Noda, T., and Shibuya, M. Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice. Proc. Natl. Acad. Sci. USA,95:9349 –9354,1998 .[Abstract/Free Full Text]
  8. Hiratsuka, S., Maru, Y., Okada, A., Seiki, M., Noda, T., and Shibuya, M. Involvement of Flt-1 tyrosine kinase (vascular endothelial growth factor receptor-1) in pathological angiogenesis. Cancer Res.,61:1207 –1213.,2001 .[Abstract/Free Full Text]
  9. Carmeliet, P., Moons, L., Luttun, A., Vincenti, V., Compernolle, V., De Mol, M., Wu, Y., Bono, F., Devy, L., Beck, H., Scholz, D., Acker, T., DiPalma, T., Dewerchin, M., Noel, A., Stalmans, I., Barra, A., Blacher, S., Vandendriessche, T., Ponten, A., Eriksson, U., Plate, K. H., Foidart, J. M., Schaper, W., Charnock-Jones, D. S., Hicklin, D. J., Herbert, J. M., Collen, D., and Persico, M. G. Synergism between vascular endothelial growth factor and placental growth factor contributes to angiogenesis and plasma extravasation in pathological conditions. Nat. Med.,7:575 –583,2001 .[CrossRef][Medline]
  10. Park, J. E., Chen, H. H., Winer, J., Houck, K. A., and Ferrara, N. Placenta growth factor. Potentiation of vascular endothelial growth factor bioactivity, in vitro and in vivo, and high affinity binding to Flt-1 but not to Flk-1/KDR. J. Biol. Chem.,269:25646 –25654,1994 .[Abstract/Free Full Text]
  11. Terman, B., Khandke, L., Dougher-Vermazan, M., Maglione, D., Lassam, N. J., Gospodarowicz, D., Persico, M. G., Bohlen, P., and Eisinger, M. VEGF receptor subtypes KDR and FLT1 show different sensitivities to heparin and placenta growth factor. Growth Factors,11:187 –195,1994 .[Medline]
  12. Kanno, S., Oda, N., Abe, M., Terai, Y., Ito, M., Shitara, K., Tabayashi, K., Shibuya, M., and Sato, Y. Roles of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF effects in human vascular endothelial cells. Oncogene,19:2138 –2146,2000 .[CrossRef][Medline]
  13. Hanahan, D., and Folkman, J. Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell,86:353 –364,1996 .[CrossRef][Medline]
  14. Fukumura, D., Xavier, R., Sugiura, T., Chen, Y., Park, E. C., Lu, N., Selig, M., Nielsen, G., Taksir, T., Jain, R. K., and Seed, B. Tumor induction of VEGF promoter activity in stromal cells. Cell,94:715 –725,1998 .[CrossRef][Medline]
  15. Grunstein, J., Roberts, W. G., Mathieu-Costello, O., Hanahan, D., and Johnson, R. S. Tumor-derived expression of vascular endothelial growth factor is a critical factor in tumor expansion and vascular function. Cancer Res.,59:1592 –1598,1999 .[Abstract/Free Full Text]
  16. Fidler, I. J., and Ellis, L. M. The implications of angiogenesis for the biology and therapy of cancer metastasis. Cell,79:185 –188,1994 .[CrossRef][Medline]
  17. Weidner, N., Semple, J. P., Welch, W. R., and Folkman, J. Tumor angiogenesis and metastasis-correlation in invasive breast carcinoma. N. Engl. J. Med.,324:1 –8,1991 .[Abstract]
  18. Kuwano, M., Fukushi, J., Okamoto, M., Nishie, A., Goto, H., Ishibashi, T., and Ono, M. Angiogenesis factors. Intern Med.,40:565 –572,2001 .[Medline]
  19. Karp, J. E., and Broder, S. Molecular foundations of cancer: new targets for intervention. Nat. Med.,1:309 –320,1995 .[CrossRef][Medline]
  20. Quinn, T. P., Peters, K. G., De Vries, C., Ferrara, N., and Williams, L. T. Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium. Proc. Natl. Acad. Sci. USA,90:7533 –7537,1993 .[Abstract/Free Full Text]
  21. Fong, T. A., Shawver, L. K., Sun, L., Tang, C., App, H., Powell, T. J., Kim, Y. H., Schreck, R., Wang, X., Risau, W., Ullrich, A., Hirth, K. P., and McMahon, G. SU5416 is a potent and selective inhibitor of the vascular endothelial growth factor receptor (Flk-1/KDR) that inhibits tyrosine kinase catalysis, tumor vascularization, and growth of multiple tumor types. Cancer Res.,59:99 –106,1999 .[Abstract/Free Full Text]
  22. Shaheen, R. M., Davis, D. W., Liu, W., Zebrowski, B. K., Wilson, M. R., Bucana, C. D., McConkey, D. J., McMahon, G., and Ellis, L. M. Antiangiogenic therapy targeting the tyrosine kinase receptor for vascular endothelial growth factor receptor inhibits the growth of colon cancer liver metastasis and induces tumor and endothelial cell apoptosis. Cancer Res.,59:5412 –5416,1999 .[Abstract/Free Full Text]
  23. Laird, A. D., Vajkoczy, P., Shawver, L. K., Thurnher, A., Liang, C., Mohammadi, M., Schlessinger, J., Ullrich, A., Hubbard, S. R., Blake, R. A., Fong, T. A., Strawn, L. M., Sun, L., Tang, C., Hawtin, R., Tang, F., Shenoy, N., Hirth, K. P., and McMahon, G. Cherrington SU6668 is a potent antiangiogenic and antitumor agent that induces regression of established tumors. Cancer Res.,60:4152 –4160,2000 .[Abstract/Free Full Text]
  24. Takahashi, T., and Shibuya, M. The 230 kDa mature form of KDR/Flk-1 (VEGF receptor-2) activates the PLC-{gamma} pathway and partially induces mitotic signals in NIH3T3 fibroblasts. Oncogene,14:2079 –2089,1997 .[CrossRef][Medline]
  25. Yamane, A., Seetharam, L., Yamaguchi, S., Gotoh, N., Takahashi, T., Neufeld, G., and Shibuya, M. A new communication system between hepatocytes and sinusoidal endothelial cells in liver through vascular endothelial growth factor and Flt tyrosine kinase receptor family (Flt-1 and KDR/Flk-1). Oncogene,9:2683 –2690,1994 .[Medline]
  26. Abe, T., Okamura, K., Ono, M., Kohno, K., Mori, T., Hori, S., and Kuwano, M. Induction of vascular endothelial tubular morphogenesis by human glioma cells. A model system for tumor angiogenesis. J. Clin. Investig.,92:54 –61,1993 .
  27. Ono, M., Kawahara, N., Goto, D., Wakabayashi, Y., Ushiro, S., Yoshida, S., Izumi, H., Kuwano, M., and Sato, Y. Inhibition of tumor growth and neovascularization by an anti-gastric ulcer agent, irsogladine. Cancer Res.,56:1512 –1516,1996 .[Abstract/Free Full Text]
  28. Nishioka, Y., Yano, S., Fujiki, F., Mukaida, N., Matsushima, K., Tsuruo, T., and Sone, S. Combined therapy of multidrug-resistant human lung cancer with anti-P-glycoprotein antibody and monocyte chemoattractant protein-1 gene transduction: the possibility of immunological overcoming of multidrug resistance. Int. J. Cancer,71:170 –177,1997 .[CrossRef][Medline]
  29. Yano, S., Sone, S., Nishioka, Y., Mukaida, N., Matsushima, K., and Ogura, T. Differential effects of anti-inflammatory cytokines (IL-4, IL-10 and IL-13) on tumoricidal and chemotactic properties of human monocytes induced by monocyte chemotactic and activating factor. J. Leukoc. Biol.,57:303 –309,1995 .[Abstract]
  30. Kundra, V., Anand-Apte, B., Feig, L. A., and Zetter, B. R. The chemotactic response to PDGF-BB: evidence of a role for Ras. J. Cell Biol.,130:725 –731,1995 .[Abstract/Free Full Text]
  31. Tsujii, M., Kawano, S., Tsuji, S., Sawaoka, H., Hori, M., and DuBois, R. N. Cyclooxygenase regulates angiogenesis induced by colon cancer cells. Cell,93:705 –716,1998 .[CrossRef][Medline]
  32. Shono, T., Motoyama, M., Tatsumi, K., Ulbrich, N., Iwamoto, Y., Kuwano, M., and Ono, M. A new synthetic matrix metalloproteinase inhibitor modulates both angiogenesis and urokinase type plasminogen activator activity. Angiogenesis,2:319 –329,1998 .[CrossRef][Medline]
  33. Sawano, A., Takahashi, T., Yamaguchi, S., Aonuma, M., and Shibuya, M. Flt-1 but not KDR/Flk-1 tyrosine kinase is a receptor for placenta growth factor, which is related to vascular endothelial growth factor. Cell Growth Differ.,7:213 –221,1996 .[Abstract]
  34. Clauss, M., Weich, H., Breier, G., Knies, U., Rockl, W., Waltenberger, J., and Risau, W. The vascular endothelial growth factor receptor Flt-1 mediates biological activities. Implications for a functional role of placenta growth factor in monocyte activation and chemotaxis. J. Biol. Chem.,271:17629 –17634,1996 .[Abstract/Free Full Text]
  35. Ziche, M., Maglione, D., Ribatti, D., Morbidelli, L., Lago, C. T., Battisti, M., Paoletti, I., Barra, A., Tucci, M., Parise, G., Vincenti, V., Granger, H. J., Viglietto, G., and Persico, M. G. Placenta growth factor-1 is chemotactic, mitogenic, and angiogenic. Lab. Investig.,76:517 –531,1997 .[Medline]
  36. Sunderkotter, C., Steinbrink, K., Goebeler, M., Bhardwaj, R., and Sorg, C. Macrophages and angiogenesis. J. Leukoc. Biol.,55:410 –422,1994 .[Abstract]
  37. Polverini, P. J. Role of the macrophage in angiogenesis-dependent diseases. EXS,79:11 –28,1997 .[Medline]
  38. Ono, M., Torisu, H., Fukushi, J., Nishie, A., and Kuwano, M. Biological implications of macrophage infiltration in human tumor angiogenesis. Cancer Chemother. Pharmacol.,43:S69 –S71,1999 .
  39. Leek, R. D., Lewis, C. E., Whitehouse, R., Greenall, M., Clarke, J., and Harris, A. L. Association of macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma. Cancer Res.,56:4625 –4629,1996 .[Abstract/Free Full Text]
  40. Nishie, A., Ono, M., Shono, T., Fukushi, J., Otsubo, M., Onoue, H., Ito, Y., Inamura, T., Ikezaki, K., Fukui, M., Iwaki, T., and Kuwano, M. Macrophage infiltration and heme oxygenase-1 expression correlate with angiogenesis in human gliomas. Clin. Cancer Res.,5:1107 –1113,1999 .[Abstract/Free Full Text]
  41. Nishie, A., Masuda, K., Otsubo, M., Migita, T., Tsuneyoshi, M., Kohno, K., Shuin, T., Naito, S., Ono, M., and Kuwano, M. High expression of the cap43 gene in infiltrating macrophages of human renal cell carcinomas. Clin. Cancer Res.,7:2145 –2151,2001 .[Abstract/Free Full Text]
  42. Torisu, H., Ono, M., Kiryu, H., Furue, M., Ohmoto, Y., Nakayama, J., Nishioka, Y., Sone, S., and Kuwano, M. Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma: possible involvement of TNF{alpha} and IL-1{alpha}. Int. J. Cancer,85:182 –188,2000 .[Medline]
  43. Toi, M., Ueno, T., Matsumoto, H., Saji, H., Funata, N., Koike, M., and Tominaga, T. Significance of thymidine phosphorylase as a marker of protumor monocytes in breast cancer. Clin. Cancer Res.,5:1131 –1137,1999 .[Abstract/Free Full Text]
  44. Torisu, H., Furue, M., Kuwano, M., and Ono, M. Co-expression of thymidine phosphorylase and Heme Oxygenase-1 in macrophages in human malignant vertical growth melanomas. Jpn. J. Cancer Res.,91:1 –5,2000 .[CrossRef][Medline]



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