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Fig. 7. NOSII expression was increased when 4-HPR was combined with IFN- or TAM. T47D cells were plated (1 x 105 cells/well) in 6-well plates in DMEM/F12 medium supplemented with 5% FBS. Cells were treated with 4-HPR (1 µM) alone or in combination with IFN- (50 IU/ml) or TAM (1 µM). Cells treated with 4-HPR alone or in combination with TAM were incubated for 5 days, and cells treated with a combination of 4-HPR and IFN- were incubated for 4 days. Cells were harvested and immunostained for NOS isoforms. A, NOSII immunostainings; B, NOSIII immunostainings. Untreated (i); 1 µM 4-HPR (ii); IFN- (iii); IFN- and 4-HPR (iv); TAM; (v); TAM and 4-HPR (vi).
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